matlab®-algorithm-based video analysis Search Results


96
MathWorks Inc r2017b
R2017b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r2017b/product/MathWorks Inc
Average 96 stars, based on 1 article reviews
r2017b - by Bioz Stars, 2026-05
96/100 stars
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96
MathWorks Inc r2015b
R2015b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
r2015b - by Bioz Stars, 2026-05
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MathWorks Inc dart interface
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Dart Interface, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
dart interface - by Bioz Stars, 2026-05
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90
Sony 4k fdr-ax100e handycam
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
4k Fdr Ax100e Handycam, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
4k fdr-ax100e handycam - by Bioz Stars, 2026-05
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Analysis GmbH intelligent video analysis &
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Intelligent Video Analysis &, supplied by Analysis GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
intelligent video analysis & - by Bioz Stars, 2026-05
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Noldus Information Technology ethovision 3.1 video analysis system
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Ethovision 3.1 Video Analysis System, supplied by Noldus Information Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethovision 3.1 video analysis system/product/Noldus Information Technology
Average 90 stars, based on 1 article reviews
ethovision 3.1 video analysis system - by Bioz Stars, 2026-05
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Hamamatsu video image analysis system aquacosmos
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Video Image Analysis System Aquacosmos, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/video image analysis system aquacosmos/product/Hamamatsu
Average 90 stars, based on 1 article reviews
video image analysis system aquacosmos - by Bioz Stars, 2026-05
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Redlake Imaging video analysis software redlake imaging motionscope
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Video Analysis Software Redlake Imaging Motionscope, supplied by Redlake Imaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/video analysis software redlake imaging motionscope/product/Redlake Imaging
Average 90 stars, based on 1 article reviews
video analysis software redlake imaging motionscope - by Bioz Stars, 2026-05
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Noldus Information Technology video analysis software noldus observer xt 10.0
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Video Analysis Software Noldus Observer Xt 10.0, supplied by Noldus Information Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/video analysis software noldus observer xt 10.0/product/Noldus Information Technology
Average 90 stars, based on 1 article reviews
video analysis software noldus observer xt 10.0 - by Bioz Stars, 2026-05
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Sony video camera sony dcr sr8e
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Video Camera Sony Dcr Sr8e, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
video camera sony dcr sr8e - by Bioz Stars, 2026-05
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Stoelting inc any-maze video tracking system v.6.32
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
Any Maze Video Tracking System V.6.32, supplied by Stoelting inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/any-maze video tracking system v.6.32/product/Stoelting inc
Average 90 stars, based on 1 article reviews
any-maze video tracking system v.6.32 - by Bioz Stars, 2026-05
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90
Carl Zeiss fully automated computer assisted video imaging analysis system
(A-G). The Drosophila <t>Arousal</t> <t>Tracking</t> <t>(DART)</t> was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.
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(A-G). The Drosophila Arousal Tracking (DART) was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.

Journal: bioRxiv

Article Title: Neurofibromin 1 mediates sleep depth in Drosophila

doi: 10.1101/2022.09.15.508161

Figure Lengend Snippet: (A-G). The Drosophila Arousal Tracking (DART) was used to probe arousal threshold and reactivity measurements. This system records fly movement while simultaneously controlling mechanical stimuli via a digital analog converter (DAC). All measurements included were taken from sleeping flies and determined hourly, starting at ZT0. A. Mechanical stimuli of increasing strength were used to assess arousal threshold and was determined by fly movement within 15 sec of stimulus delivery. B. Mechanical stimuli at maximum intensity was used to measure reactivity. A fly was considered reactive if it moved within 60 sec of stimulus delivery. C. There is a significant effect of genotype on arousal threshold (REML: F 2,94 = 37.62, P <0.0001; N = 29–35). Compared to control and heterozygote flies, arousal threshold significantly decreases in Nf1 P1 mutants and occurs during the day (+, P <0.0001; het, P <0.0001) and night (+, P <0.0001; het, P <0.0001). D. There is a significant effect of genotype on arousal threshold (REML: F 2,98 = 7.795, P <0.0007; N = 25–40). Compared to controls, panneuronal knockdown of Nf1 has mixed effects on arousal threshold during the day (nsyb>+, P <0.2138; Nf1 RNAi >+, P <0.0040) and significantly decreases arousal threshold during the night (nsyb>+, P <0.0271; Nf1 >+, P <0.00062). E. Linear regression of daytime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are not significantly different from each other (ANCOVA with time asleep as the covariate: F 2,2062 = 0.0254, P <0.9749). F. Linear regression of nighttime reactivity as a function of time asleep in Nf1 P1 mutants, heterozygotes, and their respective control. The slopes of each regression line are significantly different from each other (F 2,2100 = 57.05, P <0.0001). G. Linear regression of daytime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are not significantly different from each other (F 2,1285 = 0.5551, P <0.5741). H. Linear regression of nighttime reactivity as a function of time asleep in pan-neuronal Nf1 RNAi knockdown flies and their controls. The slopes of each regression line are significantly different from each other (F 2,1564 = 4.887, P <0.0077). For arousal threshold measurements, the median (solid line), as well as the 25th and 75th percentiles (dotted lines) are shown. For reactivity, error bars indicate ± SEM. The P -values in each panel indicates whether the slope of the regression line is significantly different from zero. White background indicates daytime, while gray background indicates nighttime. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001.

Article Snippet: The vibrational stimulus, video tracking parameters, and data analysis were performed using the DART interface developed in MATLAB (MathWorks, Natick, Massachusetts).

Techniques: